#Nisha sarang ass software#

In this respect, various bioactive molecules, including terpenoids, flavonoids, and phenolic acids, exhibit numerous anti-QS mechanisms via the inhibition of auto-inducer releases, sequestration of QS-mediated molecules, and deregulation of QS gene expression. Consequently, inhibition of QS or quorum quenching is a promising strategy to not only overcome the resistance problems but also to treat infections. Researchers demonstrated that this resistance to antibiotics is linked to a microbial cell-to-cell communication system called quorum sensing (QS). Furthermore, recent investigations showed that some bacterial strains resist these different mechanisms of antibacterial agents.

Research findings revealed that these bioactive compounds can inhibit the synthesis of DNA and proteins, block oxidative respiration, increase membrane permeability, and decrease membrane integrity. These molecules exert several mechanisms of action at different structural, cellular, and molecular levels, which could make them candidates or lead compounds for developing natural antibiotics. Recently, researchers conducted several studies to identify natural bioactive compounds, particularly secondary metabolites of medicinal plants, such as terpenoids, flavonoids, and phenolic acids, as antibacterial agents. However, compound 2 binds only three residues, so that 2 is the non-competitive inhibitor.Ĭompound 2 can be a lead compound for an antibacterial agent in each pathway.īacterial strains have developed an ability to resist antibiotics via numerous mechanisms. In nearly all proteins, biflavonoid (2) has the strongest binding affinity. Most compounds have Pa value over 0.5 against proteins in the cell wall.

#Nisha sarang ass software#

The docking results were analyzed by the ProteinsPlus and Discovery Studio software applications. Those proteins are penicillin-binding protein (PBP), MurB, Sortase A (SrtA), deoxyribonucleic acid (DNA) gyrase, ribonucleic acid (RNA) polymerase, ribosomal protein, Cytolysin M (ClyM), FsrB, gelatinase binding-activating pheromone (GBAP), and PgrX retrieved from UniProt. They were docked with 12 proteins by AutoDock Vina in the PyRx 0.8 software application.

To screen the bioactivity of compounds, ligands were analyzed by Prediction of Activity Spectra for Substances (PASS) program. Antibiotics or substrates in each protein were used as a positive control. This study aims to investigate and predict the action mode of antibacterial compounds with specific proteins by following the molecular docking approach.īutein (1), biflavonoid (2), 3″-methoxyepicatechin-3-O-epicatechin (3), 2-dodecyl-4-hydroxylbenzaldehyde (4), 2-dodecyl-4-hydroxylbenzaldehyde (5), pomolic acid (6), betulin (7), and sitosterol-(6'-O-tridecanoil)-3-O-β-D-glucopyranoside (8) from M. However, specific proteins inhibited by them have not yet been identified as either proteins targeted by antibiotics or proteins that have a role in the quorum-sensing system.

Previous research revealed that Myrmecodia pendans (Sarang Semut) contain potential antibacterial agents. Antibiotic resistance encourages the development of new therapies, or the discovery of novel antibacterial agents.